Human F12 / Factor XII

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity absorbed

Unmodified

Human Factor XII

Human F12 / Factor XII. The defined antibody reactivity is restricted to FXII, FXIIa and complexes of FXIIa with other proteins. In immunoelectrophoresis, bi-dimensional electrophoresis and radial immunodiffusion (Ouchterlony) against normal plasma, a single precipitin line is obtained which shows a reaction of identity with precipitated purified Factor XII. No reaction is obtained with FXII-depleted plasma. Cross-reactivity The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in immunoelectrophoresis a reaction with Rhesus monkey plasma has been found.

• Immunodiffusion

This antiserum is primarily intended for the measurement of Factor XII in human plasma using the EID-method (Laurell): a specially purified agarose solution containing between 1 and 2% antiserum is poured onto a glass plate and allowed to gel. Plasma containing FXII is incorporated into a series of wells and electrophoresis is performed. Rockets due to antigen/antibody reaction are measured. The height of the rocket is directly proportional to the FXII concentration in the plasma.

Lyophilized antiserum

Use 1 ml of sterile Distilled water.

Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P00748 NCBI: NM_000505 NP_000496.2