Target
Human EWSR1 / EWS
Synonyms
EWSR1 | EWS | RNA-binding protein EWS | BK984G1.4 | EWS oncogene
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig, Cow (at least 90% immunogen sequence identity)
Purification
ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Immunogen
TSYDQSSYSQQNTYG
Specificity
This antibody is expected to recognize all five reported isoforms (NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1).
Applications
- Western blot
- Peptide Enzyme-Linked Immunosorbent Assay
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 90kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 68.5kDa according to NP_053733.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee