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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ETV5 / ERM Antibody (clone 3B10) LS‑C197166
ERM antibody LS-C197166 is an unconjugated mouse monoclonal antibody to human ERM (ETV5). Validated for ELISA and WB.
Catalog
Size
Price
LS-C197166-100
100 µg
$355
Description
ERM antibody LS-C197166 is an unconjugated mouse monoclonal antibody to human ERM (ETV5). Validated for ELISA and WB.
Target
Human ETV5 / ERM
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [3B10]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
ETV5 / ERM antibody was raised against eTV5 (NP_004445, aa181-290 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human ETV5 / ERM
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About ETV5 / ERM
P41161 NM_004454 NP_004445.1

Popular ETV5 / ERM Products

Anti-ETV5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Anti-ETV5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Western blot of ETV5 Antibody antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in SW480 cell line lysate. ETV5 Antibody (arrow) was detected using the purified antibody.
Species: Human
Applications: Western blot, Flow Cytometry
Anti-ETV5 / ERM antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

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Images

Western blot

ETV5 monoclonal antibody (M01), clone 3B10 Western Blot analysis of ETV5 expression in HeLa NE.
ETV5 monoclonal antibody (M01), clone 3B10 Western Blot analysis of ETV5 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ETV5 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ETV5 is approximately 0.3 ng/ml as a capture antibody.

Western blot

ETV5 monoclonal antibody (M01), clone 3B10 Western Blot analysis of ETV5 expression in HeLa NE.
ETV5 monoclonal antibody (M01), clone 3B10 Western Blot analysis of ETV5 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ETV5 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ETV5 is approximately 0.3 ng/ml as a capture antibody.

Western blot

ETV5 monoclonal antibody (M01), clone 3B10 Western Blot analysis of ETV5 expression in HeLa NE.
ETV5 monoclonal antibody (M01), clone 3B10 Western Blot analysis of ETV5 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ETV5 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ETV5 is approximately 0.3 ng/ml as a capture antibody.

Western blot

ETV5 monoclonal antibody (M01), clone 3B10 Western Blot analysis of ETV5 expression in HeLa NE.
ETV5 monoclonal antibody (M01), clone 3B10 Western Blot analysis of ETV5 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ETV5 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ETV5 is approximately 0.3 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 11/20/2018
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