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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ETF1 / ERF1 Antibody (clone 2E3) LS‑C172761 Best Seller
ERF1 antibody LS-C172761 is an unconjugated mouse monoclonal antibody to human ERF1 (ETF1). Validated for Flow and WB.
Catalog
Size
Price
LS-C172761-100
100 µl (0.97 mg/ml)
$345

Popular ETF1 / ERF1 Products

Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Western blot analysis using *** mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Immunohistochemistry Image
Species: Human
Applications: IHC, Western blot, ELISA
Western Blot analysis of ETF1 expression in transfected 293T cell line by ETF1 monoclonal antibody (M02), clone 2H4.Lane 1: ETF1 transfected lysate (Predicted MW: 49 KDa).Lane 2: Non-transfected lysate.
Species: Human
Applications: Western blot, ELISA

Product Description

ERF1 antibody LS-C172761 is an unconjugated mouse monoclonal antibody to human ERF1 (ETF1). Validated for Flow and WB.

Specifications

Target
Human ETF1 / ERF1
Synonyms
ETF1, D5S1995, ERF1, Eukaryotic release factor 1, Protein Cl1, RF1, SUP45L1, TB3-1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [2E3]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Western blot (1:500)
  • Flow Cytometry (1:100)
Immunogen
ETF1 / ERF1 antibody was raised against full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.
Specificity
Human ETF1 / ERF1
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ETF1 / ERF1
Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. P62495 NM_004730 NP_004721.1


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Images

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-ETF1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Requested From: United States
Date Requested: 11/13/2018

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