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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ESRRA / ERR Alpha Antibody (clone 6F1E6) LS‑C682397
ERR Alpha antibody LS-C682397 is an unconjugated mouse monoclonal antibody to human ERR Alpha (ESRRA). Validated for ELISA, Flow and WB.
Catalog
Size
Price
LS-C682397-100
100 µl
$365

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Product Description

ERR Alpha antibody LS-C682397 is an unconjugated mouse monoclonal antibody to human ERR Alpha (ESRRA). Validated for ELISA, Flow and WB.

Specifications

Target
Human ESRRA / ERR Alpha
Synonyms
ESRRA, ERRa, ERR1, HERR1, NR3B1, Steroid hormone receptor ERR1, ERR-alpha, ERRalpha, ESRL1, Estrogen receptor-like 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [6F1E6]
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • Western blot
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Immunogen
Purified recombinant fragment of human ESRRA (AA: 198-376) expressed in E. Coli.
Usage
Predicted Western blot MW: 45.5kDa
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
About ESRRA / ERR Alpha
Estrogen-related receptor alpha (ERR alpha), a NR3 Steroid Receptor, was isolated based on sequence similarity in its DNA-binding domain to estrogen receptor alpha (ER alpha). ERR alpha has been shown to regulate the promoters of lactoferrin, medium-chain acyl CoA dehydrogenase, osteopontin, and thyroid receptor alpha, and it may affect cellular energy balance and bone formation. P11474 NM_004451 NP_004442.3


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Images

Western blot

Western blot analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot analysis using ESRRA mouse mAb against C6 (1) cell lysate.
Western blot analysis using ESRRA mouse mAb against C6 (1) cell lysate.

Western blot

Western blot analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)
Western blot analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)

Flow Cytometry

Flow cytometric analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).

Enzyme-Linked Immunosorbent Assay

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot

Western blot analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot analysis using ESRRA mouse mAb against C6 (1) cell lysate.
Western blot analysis using ESRRA mouse mAb against C6 (1) cell lysate.

Western blot

Western blot analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)
Western blot analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)

Flow Cytometry

Flow cytometric analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).

Enzyme-Linked Immunosorbent Assay

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot

Western blot analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot analysis using ESRRA mouse mAb against C6 (1) cell lysate.
Western blot analysis using ESRRA mouse mAb against C6 (1) cell lysate.

Western blot

Western blot analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)
Western blot analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)

Flow Cytometry

Flow cytometric analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).

Enzyme-Linked Immunosorbent Assay

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot

Western blot analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot analysis using ESRRA mouse mAb against C6 (1) cell lysate.
Western blot analysis using ESRRA mouse mAb against C6 (1) cell lysate.

Western blot

Western blot analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)
Western blot analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)

Flow Cytometry

Flow cytometric analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).

Enzyme-Linked Immunosorbent Assay

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Requested From: United States
Date Requested: 11/15/2018

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