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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ESRP1 / RBM35A Antibody (clone 27H12) LS‑C154126

Catalog Number Size Price
LS-C154126-100 100 µg (1 mg/ml) $425 
Anti-ESRP-1 Antibody - Western Blot. Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.

ESRP1 / RBM35A Antibody (clone 27H12) LS‑C154126

Antibody:
Mouse Monoclonal to Mouse ESRP1 / RBM35A
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154126-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Mouse ESRP1 / RBM35A
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
RBM35A antibody LS-C154126 is an unconjugated mouse monoclonal antibody to mouse RBM35A (ESRP1 ). Validated for ELISA and WB. Cited in 1 publication.
Target
Mouse ESRP1 / RBM35A
Host
Mouse
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
27H12
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA
Immunogen
Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
Specificity
This antibody reacts with mouse Esrp-1 protein. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
Usage
This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kD in size corresponding to Esrp-1 by western blotting in the appropriate cell lysate or extract.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ESRP1 / RBM35A
Q6NXG1 NM_017697 NP_060167.2

Publications (1)

An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. Warzecha CC, Jiang P, Amirikian K, Dittmar KA, Lu H, Shen S, Guo W, Xing Y, Carstens RP. The EMBO journal. 2010 29:3286-300.

Reviews (0)

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HCT116 cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Pig, Rabbit, Chicken
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ESRP1 antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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Range: 15.6-1000 pg/ml

Requested From: United States
Date Requested: 8/18/2019
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