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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C154126-100 100 µg (1 mg/ml) $425 
ESRP1 / RBM35A Antibody - Anti-ESRP-1 Antibody - Western Blot. Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.

Monoclonal Mouse anti‑Mouse ESRP1 / RBM35A Antibody (clone 27H12.F3.H5, WB) LS‑C154126

Monoclonal Mouse anti‑Mouse ESRP1 / RBM35A Antibody (clone 27H12.F3.H5, WB) LS‑C154126

Antibody:
Mouse Monoclonal to Mouse ESRP1 / RBM35A
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154126-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Mouse ESRP1 / RBM35A
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
RBM35A antibody LS-C154126 is an unconjugated mouse monoclonal antibody to mouse RBM35A (ESRP1). Validated for ELISA and WB. Cited in 1 publication.
Target
Mouse ESRP1 / RBM35A
Synonyms
ESRP1 | RBM35A | RMB35A | RNA binding motif protein 35A | RNA-binding motif protein 35A | RNA-binding protein 35A
Host
Mouse
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Rat, Bovine, Dog, Horse, Opossum, Panda, Pig (at least 90% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
27H12.F3.H5
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA (1:750000)
Immunogen
Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
Specificity
This antibody reacts with mouse Esrp-1 protein. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
Usage
This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kD in size corresponding to Esrp-1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ESRP1 / RBM35A
Q6NXG1 NM_017697 NP_060167.2

LSBio Ratings

ESRP1 / RBM35A Antibody (clone 27H12.F3.H5) for WB/Western, ELISA LS-C154126 has an LSBio Rating of
Publications (4)

Publications (1)

An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. Warzecha CC, Jiang P, Amirikian K, Dittmar KA, Lu H, Shen S, Guo W, Xing Y, Carstens RP. The EMBO journal. 2010 29:3286-300.

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Requested From: United States
Date Requested: 5/30/2020