ESR2 / ER Beta antibody was raised against synthetic peptide corresponding to aa 100-115 (A100EPQKSPWCEARSLEH115) of human, mouse and rat ER (estrogen receptor). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Bat (100%); Sheep, Dog (94%); Panda, Bovine, Horse, Pig, Opossum (88%).
Recognizes human, mouse, rat and non-human primate ER. Detects a band of ~55kD by Western blot. Does not cross-react with ER.
Gel Shift Assay (1-5 ug/ml). Immunohistochemistry ( (frozen sections, paraffin sections (1-2 ug/ml)). Western Blot (1-2 ug/ml).
PBS, 1 mg/ml BSA, 0.05% Sodium Azide
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.