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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ERCC6 / CSB Antibody (aa784‑833) LS‑C201334
CSB antibody LS-C201334 is an unconjugated rabbit polyclonal antibody to CSB (ERCC6) from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C201334-50
50 µg
$365

Popular ERCC6 / CSB Products

Antibody
Species: Human
Applications: Western blot
Antibody
Species: Human
Applications: Western blot, ELISA
Immunohistochemical analysis of ERCC6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-CSB / ERCC6 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin
Antibody
Species: Human
Applications: IHC, ELISA

Product Description

CSB antibody LS-C201334 is an unconjugated rabbit polyclonal antibody to CSB (ERCC6) from human and monkey. Validated for WB.

Specifications

Target
Human ERCC6 / CSB
Synonyms
ERCC6, ARMD5, Cockayne syndrome B protein, Cockayne syndrome protein CSB, COFS, CSB, Rad26 homolog, ATP-dependent helicase ERCC6, CKN2, COFS1, RAD26, UVSS1
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ERCC6 / CSB antibody was raised against synthetic peptide located between aa784-833 of human ERCC6 (F5H493). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%); Galago (92%); Bovine, Horse, Guinea pig (85%); Mouse, Rat (78%).
Epitope
aa784-833
Specificity
Human CSB / ERCC6
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ERCC6 / CSB
Essential factor involved in transcription-coupled nucleotide excision repair which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA. It is required for transcription-coupled repair complex formation. Q03468 NM_000124 NP_000115.1


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Images

Western blot

Western blot of Human HepG2. ERCC6 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human HepG2. ERCC6 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human HepG2. ERCC6 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human HepG2. ERCC6 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human HepG2. ERCC6 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human HepG2. ERCC6 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human HepG2. ERCC6 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human HepG2. ERCC6 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/13/2018

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