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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2815-50 | 50 µg (1 mg/ml) | $615 |
IHC‑plus™ Monoclonal Mouse anti‑Human ERCC3 / XPB Antibody (clone 3G4, aa242‑261, IHC, WB) LS‑B2815
IHC‑plus™ Monoclonal Mouse anti‑Human ERCC3 / XPB Antibody (clone 3G4, aa242‑261, IHC, WB) LS‑B2815
Note: This antibody replaces LS-C6713
Antibody:
ERCC3 / XPB Mouse anti-Human Monoclonal (aa242-261) (3G4) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ERCC3 / XPB Mouse anti-Human Monoclonal (aa242-261) (3G4) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
XPB antibody LS-B2815 is an unconjugated mouse monoclonal antibody to human XPB (ERCC3) (aa242-261). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ERCC3 / XPB
Synonyms
ERCC3 | BTF2 | GTF2H | RAD25 | TFIIH p89 | TFIIH | XPB | BTF2 p89 | XPBC | TFIIH 89 kDa subunit
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Clone
3G4
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa242-261 of human ERCC-3.
Epitope
aa242-261
Specificity
Recognizes an epitope located between aa242-261 of ERCC-3 (largest subunit of TFIIH).
Applications
- IHC
- IHC - Paraffin (5 - 10 µg/ml)
- Western blot
- Immunoprecipitation
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2815 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2815 was determined to be 5-10 ug/ml. Immunoprecipitation: Immunoprecipitates transcriptionally active TFIIH in a reconstituted assay.
Presentation
0.1 M Tris-HCl, pH 7.5
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ERCC3 / XPB
Validation
Anti-ERCC3 / XPB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ERCC3 / XPB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ERCC3 / XPB Antibody (aa242-261, clone 3G4) for IHC, WB/Western, IP, ELISA LS-B2815 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Reactivity:
Human
Range:
3.12-200 ng/ml
Reactivity:
Human
Range:
15.6-1000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
