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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C12217-500 500 µl $680 
Antibody

Monoclonal Mouse anti‑Human ERBB2 / HER2 Antibody (Cytoplasmic Domain, IHC, WB) LS‑C12217

Monoclonal Mouse anti‑Human ERBB2 / HER2 Antibody (Cytoplasmic Domain, IHC, WB) LS‑C12217

Antibody:
Mouse Monoclonal to Human ERBB2 / HER2
Application:
IHC, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$680 
LS-C12217-500
500 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human ERBB2 / HER2
Application:
IHC, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HER2 antibody LS-C12217 is an unconjugated mouse monoclonal antibody to HER2 (ERBB2) (Cytoplasmic Domain) from human. It is reactive with human, mouse and rat. Validated for IHC, IP and WB.
Target
Human ERBB2 / HER2
Synonyms
ERBB2 | C-erb B2/neu protein | CD340 | HER2 | Herstatin | MLN 19 | HER-2 | Neu | NGL | Proto-oncogene c-ErbB-2 | Proto-oncogene Neu | CD340 antigen | MLN19 | p185erbB2 | TKR1
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC (2 - 4 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Cytoplasmic domain of recombinant human c-erbB-2/HER-2 oncoprotein. MW of Antigen: 185kD. Epitope: Cytoplasmic domain. Cellular Localization: Cell membrane.
Epitope
Cytoplasmic Domain
Specificity
Recognizes human c-erbB-2 (HER-2, neu). Species cross-reactivity: Mouse and rat.
Usage
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and Denatured) (Use Protein G): 2 ug/mg protein lysate. Western Blot: 1-2 ug/ml for 2hr at RT. Immunohistochemistry: 2-4 ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: SKBR-3 or T47D cells, or breast carcinoma.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ERBB2 / HER2
P04626 NM_004448 NP_004439.2

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Requested From: United States
Date Requested: 7/11/2020