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EPHA4 / EPH Receptor A4 Antibody (aa40‑70) LS‑C100259

Catalog Number Size Price
LS-C100259-400 400 µl $325 
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Methanol/Acetone fixed human stem cell is used in IF to detect Eph4A (blue) and endothelial Lectin(red). Data kindly provided by Dr. Weis from Cheresh Lab, UCSD.
Western blot of anti-EphA4 N-term antibody in HeLa cell lysate. EphA4 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of EphA4 (arrow) using EphA4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA4 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Methanol/Acetone fixed human stem cell is used in IF to detect Eph4A (blue) and endothelial Lectin(red). Data kindly provided by Dr. Weis from Cheresh Lab, UCSD.
Western blot of anti-EphA4 N-term antibody in HeLa cell lysate. EphA4 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of EphA4 (arrow) using EphA4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA4 gene (Lane 2) (Origene Technologies).
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EPHA4 / EPH Receptor A4 Antibody (aa40‑70) LS‑C100259

Antibody:
Rabbit Polyclonal to Human EPHA4 / EPH Receptor A4
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C100259-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human EPHA4 / EPH Receptor A4
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EPH Receptor A4 antibody LS-C100259 is an unconjugated rabbit polyclonal antibody to human EPH Receptor A4 (EPHA4 ). Validated for Flow, IF, IHC and WB.
Target
Human EPHA4 / EPH Receptor A4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9102 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C100259. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa40-70
Specificity
This EphA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 amino acids from the N-terminal region of human EphA4.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EPHA4 / EPH Receptor A4
P54764 NM_004438 NP_004429.1

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Requested From: United States
Date Requested: 9/16/2019