Human EPHA2 / EPH Receptor A2
EPHA2 | CTPA | EPH receptor A2 | Ephrin type-A receptor 2 | Epithelial cell kinase | ECK | Sek-2 | ARCC2 | Soluble EPHA2 variant 1 | CTPP1
Human, Mouse, Bovine, Dog
(tested or 100% immunogen sequence identity)
Protein G purified
NS0-derived rhEphA2 extracellular domain.
Recognizes human EphA2.
- ICC (10 µg/ml)
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (25 µg/ml)
Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Flow Cytometry: 25 ug/ml. Add 10 ul to label 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. Western Blot: 1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhEphA2 is approximately 1 ng/lane under non-reducing conditions. Immunocytochemistry: 10 ug/ml to detect EphA2 on A431 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4°C followed by Rhodamine RedTM-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitute in 200 ul sterile PBS
Store at -20°C lyophilized. Once reconstituted: Store at -20°C. Aliquot to avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.
About EPHA2 / EPH Receptor A2