Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C36249-100 100 µg $747 
Antibody

Monoclonal Mouse anti‑Human EPHA2 / EPH Receptor A2 Antibody (aa25‑534, WB) LS‑C36249

Monoclonal Mouse anti‑Human EPHA2 / EPH Receptor A2 Antibody (aa25‑534, WB) LS‑C36249

Antibody:
EPHA2 / EPH Receptor A2 Mouse anti-Human Monoclonal (aa25-534) Antibody
Application:
ICC, WB, Flo
Reactivity:
Human, Mouse, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$747
LS-C36249-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
EPHA2 / EPH Receptor A2 Mouse anti-Human Monoclonal (aa25-534) Antibody
Application:
ICC, WB, Flo
Reactivity:
Human, Mouse, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
EPH Receptor A2 antibody LS-C36249 is an unconjugated mouse monoclonal antibody to EPH Receptor A2 (EPHA2) (aa25-534) from human. It is reactive with human, mouse, bovine and other species. Validated for Flow, ICC and WB.
Target
Human EPHA2 / EPH Receptor A2
Synonyms
EPHA2 | CTPA | EPH receptor A2 | Ephrin type-A receptor 2 | Epithelial cell kinase | ECK | Sek-2 | ARCC2 | Soluble EPHA2 variant 1 | CTPP1
Host
Mouse
Reactivity
Human, Mouse, Bovine, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
NS0-derived rhEphA2 extracellular domain.
Epitope
aa25-534
Specificity
Recognizes human EphA2.
Applications
  • ICC (10 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Flow Cytometry (25 µg/ml)
Usage
Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Flow Cytometry: 25 ug/ml. Add 10 ul to label 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. Western Blot: 1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhEphA2 is approximately 1 ng/lane under non-reducing conditions. Immunocytochemistry: 10 ug/ml to detect EphA2 on A431 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4°C followed by Rhodamine RedTM-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Store at -20°C lyophilized. Once reconstituted: Store at -20°C. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EPHA2 / EPH Receptor A2
P29317 NM_004431 NP_004422.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/22/2021