EPCAM | 323/A3 | ACSTD1 | 17-1A | CD326 | EGP | EGP34 | Epithelial glycoprotein | GA733-2 | HNPCC8 | Ep-CAM | ESA | HEGP314 | KS 1/4 antigen | KS1/4 | KSA | M1S2 | MIC18 | MK-1 | MH99 | TROP1 | TACST-1 | TACSTD1 | CD326 antigen | CO-17A | DIAR5 | EGP-2 | EGP314 | EGP40 | Epithelial glycoprotein 314 | HEA125 | Ly74 | M4S1
(tested or 100% immunogen sequence identity)
Also available Unconjugated
or conjugated with Biotin
Protein A purified
Recombinant human CD326(EpCAM).
Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS.
- Flow Cytometry
- Applications tested for the base form of this product only
Cultured human SW480 cells were harvested by EDTA. Five x 105 cells per tube were pre incubated with 20 ul of 300 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of product at a 1:50 dilution (10 ug/ml). They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.30 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD326 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.