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Catalog Number Size Price
LS-C357671-120 120 Tests $476 
EPCAM Antibody - Flow cytometry of TACSTD1 / EPCAM antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Human EPCAM Antibody (clone ANC8D4, FITC) LS‑C357671

Monoclonal Mouse anti‑Human EPCAM Antibody (clone ANC8D4, FITC) LS‑C357671

Antibody:
EPCAM Mouse anti-Human Monoclonal (FITC) (ANC8D4) Antibody
Application:
Flo, ELISA
Reactivity:
Human
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$476
LS-C357671-120
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
EPCAM Mouse anti-Human Monoclonal (FITC) (ANC8D4) Antibody
Application:
Flo, ELISA
Reactivity:
Human
Format:
FITC, Unmodified
Other formats:

Specifications

Description
EPCAM antibody LS-C357671 is an FITC-conjugated mouse monoclonal antibody to human EPCAM. Validated for ELISA and Flow.
Target
Human EPCAM
Synonyms
EPCAM | 323/A3 | ACSTD1 | 17-1A | CD326 | EGP | EGP34 | Epithelial glycoprotein | GA733-2 | HNPCC8 | Ep-CAM | ESA | HEGP314 | KS 1/4 antigen | KS1/4 | KSA | M1S2 | MIC18 | MK-1 | MH99 | TROP1 | TACST-1 | TACSTD1 | CD326 antigen | CO-17A | DIAR5 | EGP-2 | EGP314 | EGP40 | Epithelial glycoprotein 314 | HEA125 | Ly74 | M4S1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
ANC8D4
Conjugations
FITC. Also available Unconjugated or conjugated with Biotin, RPE.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant human CD326(EpCAM).
Specificity
Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS.
Applications
  • Flow Cytometry
  • ELISA
  • Applications tested for the base form of this product only
Usage
Cultured human SW480 cells were harvested by EDTA. Five x 105 cells per tube were pre incubated with 20 ul of 300 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of product at a 1:50 dilution (10 ug/ml). They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.30 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD326 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Storage
Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EPCAM
P16422 NM_002354 NP_002345.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/4/2021