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Catalog Number Size Price
LS-C287259-10 10 µl $295 
LS-C287259-100 100 µl (1 mg/ml) $514 
EP300 / p300 Antibody - Detection of human p300 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-p300 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
EP300 / p300 Antibody - Detection of Human p300 by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-p300 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
EP300 / p300 Antibody - Detection of human p300 by western blot. Samples: Whole cell lysate from HeLa (15, 5 µg), HEK293T (15, 5 µg), and Jurkat (50 µg)cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p300 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
EP300 / p300 Antibody - Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
EP300 / p300 Antibody - Detection of human p300 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-p300 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
EP300 / p300 Antibody - Detection of Human p300 by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-p300 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
EP300 / p300 Antibody - Detection of human p300 by western blot. Samples: Whole cell lysate from HeLa (15, 5 µg), HEK293T (15, 5 µg), and Jurkat (50 µg)cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p300 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
EP300 / p300 Antibody - Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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Polyclonal Rabbit anti‑Human EP300 / p300 Antibody (aa950‑1000, IHC, WB) LS‑C287259

Polyclonal Rabbit anti‑Human EP300 / p300 Antibody (aa950‑1000, IHC, WB) LS‑C287259

Antibody:
EP300 / p300 Rabbit anti-Human Polyclonal (aa950-1000) Antibody
Application:
IHC, IHC-P, WB, IP, ChIP-Seq
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287259-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
EP300 / p300 Rabbit anti-Human Polyclonal (aa950-1000) Antibody
Application:
IHC, IHC-P, WB, IP, ChIP-Seq
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P300 antibody LS-C287259 is an unconjugated rabbit polyclonal antibody to human p300 (EP300) (aa950-1000). Validated for ChIP-Seq, IHC, IP and WB.
Target
Human EP300 / p300
Synonyms
EP300 | E1A binding protein p300 | Histone acetyltransferase p300 | KAT3B | p300 HAT | E1A-associated protein p300 | E1A-binding protein, 300kD | p300 | RSTS2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 2033 corresponding to amino acids between 950 and 1000 of UniprotID: Q09472
Epitope
aa950-1000
Specificity
Region between residues 950 and 1000 of human E1Aassociated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).
Applications
  • IHC
  • IHC - Paraffin (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (1 - 5 µg/mg lysate)
  • ChIP-sequencing
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ChIP-Seq: 10 µg
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EP300 / p300
Q09472 NM_001429 NP_001420.2

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Requested From: United States
Date Requested: 4/23/2024