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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C353977-100 100 µl (1 mg/ml) $265 
EIF4EBP1 / 4EBP1 Antibody - Immunohistochemical analysis of 4EBP1 (pT46) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EIF4EBP1 / 4EBP1 Antibody - Western blot analysis of 4EBP1 (pT46) expression in K562 PMA-treated (A); NIH3T3 PMA-treated (B); PC12 PMA-treated (C) whole cell lysates.
EIF4EBP1 / 4EBP1 Antibody - Immunohistochemical analysis of 4EBP1 (pT46) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EIF4EBP1 / 4EBP1 Antibody - Western blot analysis of 4EBP1 (pT46) expression in K562 PMA-treated (A); NIH3T3 PMA-treated (B); PC12 PMA-treated (C) whole cell lysates.
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EIF4EBP1 / 4EBP1 Antibody (phospho‑Thr46) LS‑C353977

EIF4EBP1 / 4EBP1 Antibody (phospho‑Thr46) LS‑C353977

Antibody:
Rabbit Polyclonal to Human EIF4EBP1 / 4EBP1
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$265 
LS-C353977-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human EIF4EBP1 / 4EBP1
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
4EBP1 antibody LS-C353977 is an unconjugated rabbit polyclonal antibody to 4EBP1 (EIF4EBP1 ) from human, mouse, rat and other species. Validated for IHC and WB.
Target
Human EIF4EBP1 / 4EBP1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human 4EBP1 (pT46).
Blocking Peptide
LS-E43782 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-C353977. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
pThr46
Specificity
Recognizes endogenous levels of 4EBP1 (pT46) protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EIF4EBP1 / 4EBP1
Q13541 NM_004095 NP_004086.1

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Requested From: United States
Date Requested: 10/18/2019