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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF4A3 Antibody (aa125‑175) IHC‑plus™ LS‑B12351
Note: This antibody replaces LS-C289688
EIF4A3 antibody LS-B12351 is an unconjugated rabbit polyclonal antibody to EIF4A3 from human, mouse, rat and other species. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B12351-50
50 µl (0.2 mg/ml)
$425

Popular EIF4A3 Products

Antibody
Species: Human
Applications: Western blot
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Chicken, Xenopus, Zebrafish
Applications: Western blot
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

EIF4A3 antibody LS-B12351 is an unconjugated rabbit polyclonal antibody to EIF4A3 from human, mouse, rat and other species. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human EIF4A3
Synonyms
EIF4A3, DDX48, DEAD box protein 48, EIF-4A-III, EIF4A-III, EIF4AIII, HNMP 265, NMP265, NMP 265, KIAA0111, Nuclear matrix protein 265, NUK34
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:250)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 5 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
aa125-175
Specificity
Region between residue 125 and 175 of human Eukaryotic Translation Initiation Factor 4A3 using the numbering given in entry NP_055555.1 (GeneID 9775).
Usage
Immunohistochemistry: Antigen retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma, Prostate Carcinoma.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.
About EIF4A3
ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. P38919 NM_014740 NP_055555.1


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Images

Immunohistochemistry - Paraffin

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Requested From: United States
Date Requested: 11/14/2018

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