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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF4A2 Antibody (aa333‑360) LS‑C167695
EIF4A2 antibody LS-C167695 is an unconjugated rabbit polyclonal antibody to human EIF4A2. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C167695-400
400 µl
$305
Description
EIF4A2 antibody LS-C167695 is an unconjugated rabbit polyclonal antibody to human EIF4A2. Validated for Flow, IHC and WB.
Target
Human EIF4A2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Rabbit, Chicken, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9995 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C167695. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa333-360
Specificity
This EIF4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-360 amino acids from the C-terminal region of human EIF4A2.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About EIF4A2
Q14240 NM_001967 NP_001958.2

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Images

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.
Western blot of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.

Western blot

Western blot of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane). EIF4A2 (arrow) was detected using the purified antibody.
Western blot of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane). EIF4A2 (arrow) was detected using the purified antibody.

Flow Cytometry

EIF4A2 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
EIF4A2 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.
Western blot of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.

Western blot

Western blot of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane). EIF4A2 (arrow) was detected using the purified antibody.
Western blot of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane). EIF4A2 (arrow) was detected using the purified antibody.

Flow Cytometry

EIF4A2 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
EIF4A2 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.
Western blot of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.

Western blot

Western blot of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane). EIF4A2 (arrow) was detected using the purified antibody.
Western blot of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane). EIF4A2 (arrow) was detected using the purified antibody.

Flow Cytometry

EIF4A2 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
EIF4A2 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.
Western blot of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.

Western blot

Western blot of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane). EIF4A2 (arrow) was detected using the purified antibody.
Western blot of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane). EIF4A2 (arrow) was detected using the purified antibody.

Flow Cytometry

EIF4A2 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
EIF4A2 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/20/2018
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