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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF4A2 Antibody (aa1‑50) LS‑C148457
EIF4A2 antibody LS-C148457 is an unconjugated rabbit polyclonal antibody to EIF4A2 from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C148457-100
100 µg
$335

Popular EIF4A2 Products

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-EIF4A antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Species: Human, Monkey, Mouse, Dog, Chicken, Macaque
Applications: IHC, IHC - Paraffin, Western blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

EIF4A2 antibody LS-C148457 is an unconjugated rabbit polyclonal antibody to EIF4A2 from human, mouse, rat and other species. Validated for IHC and WB.

Specifications

Target
Human EIF4A2
Synonyms
EIF4A2, BM-010, EIF-4A-II, EIF4A, EIF4A-II, EIF4F, DDX2B
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
EIF4A2 antibody was raised against a portion of amino acids 1-50 of human eIF4A2.
Epitope
aa1-50
Specificity
Human eIF4A2.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
Restrictions
For research use only.
About EIF4A2
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Q14240 NM_001967 NP_001958.2


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Images

Immunohistochemistry

IHC ofeIF4A2 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) and Polyclonal Antibody to eIF4A2 (bottom) at 5 ug/ml.
IHC ofeIF4A2 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) and Polyclonal Antibody to eIF4A2 (bottom) at 5 ug/ml.

Western blot

Western blot of eIF4A2 in human liver lysate in the 1) absence and2) presence of immunizing peptide and 3) mouse liver lysate using Polyclonal Antibody to eIF4A2 at 0.5 ug/ml.
Western blot of eIF4A2 in human liver lysate in the 1) absence and2) presence of immunizing peptide and 3) mouse liver lysate using Polyclonal Antibody to eIF4A2 at 0.5 ug/ml.

Immunohistochemistry

IHC ofeIF4A2 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) and Polyclonal Antibody to eIF4A2 (bottom) at 5 ug/ml.
IHC ofeIF4A2 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) and Polyclonal Antibody to eIF4A2 (bottom) at 5 ug/ml.

Western blot

Western blot of eIF4A2 in human liver lysate in the 1) absence and2) presence of immunizing peptide and 3) mouse liver lysate using Polyclonal Antibody to eIF4A2 at 0.5 ug/ml.
Western blot of eIF4A2 in human liver lysate in the 1) absence and2) presence of immunizing peptide and 3) mouse liver lysate using Polyclonal Antibody to eIF4A2 at 0.5 ug/ml.

Immunohistochemistry

IHC ofeIF4A2 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) and Polyclonal Antibody to eIF4A2 (bottom) at 5 ug/ml.
IHC ofeIF4A2 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) and Polyclonal Antibody to eIF4A2 (bottom) at 5 ug/ml.

Western blot

Western blot of eIF4A2 in human liver lysate in the 1) absence and2) presence of immunizing peptide and 3) mouse liver lysate using Polyclonal Antibody to eIF4A2 at 0.5 ug/ml.
Western blot of eIF4A2 in human liver lysate in the 1) absence and2) presence of immunizing peptide and 3) mouse liver lysate using Polyclonal Antibody to eIF4A2 at 0.5 ug/ml.

Immunohistochemistry

IHC ofeIF4A2 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) and Polyclonal Antibody to eIF4A2 (bottom) at 5 ug/ml.
IHC ofeIF4A2 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) and Polyclonal Antibody to eIF4A2 (bottom) at 5 ug/ml.

Western blot

Western blot of eIF4A2 in human liver lysate in the 1) absence and2) presence of immunizing peptide and 3) mouse liver lysate using Polyclonal Antibody to eIF4A2 at 0.5 ug/ml.
Western blot of eIF4A2 in human liver lysate in the 1) absence and2) presence of immunizing peptide and 3) mouse liver lysate using Polyclonal Antibody to eIF4A2 at 0.5 ug/ml.

Requested From: United States
Date Requested: 11/13/2018

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