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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF3I / EIF3S2 Antibody (aa128‑177, HRP) LS‑C472531
EIF3S2 antibody LS-C472531 is an HRP-conjugated rabbit polyclonal antibody to EIF3S2 (EIF3I) from human, chimpanzee, dog and other species. Validated for WB.
Catalog
Size
Price
LS-C472531-100
100 µl (0.65 mg/ml)
$385

Popular EIF3I / EIF3S2 Products

Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Western blot of Eukaryotic translation initiation factor 3, subunit 2 beta antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 36 kDa. Observed band size: 80 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot, ELISA
Western blot of Eukaryotic translation initiation factor 3, subunit 2 beta antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 36 kDa. Observed band size: 80 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot, ELISA
Western blot of Eukaryotic translation initiation factor 3, subunit 2 beta antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 36 kDa. Observed band size: 80 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot, ELISA
Western blot of Eukaryotic translation initiation factor 3, subunit 2 beta antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 36 kDa. Observed band size: 80 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

EIF3S2 antibody LS-C472531 is an HRP-conjugated rabbit polyclonal antibody to EIF3S2 (EIF3I) from human, chimpanzee, dog and other species. Validated for WB.

Specifications

Target
Human EIF3I / EIF3S2
Synonyms
EIF3I, EIF3-beta, EIF3S2, EIF-3-beta, EIF3 p36, Predicted protein of HQ2242, TRIP-1, EIF3-p36, PRO2242, TRIP1
Host
Rabbit
Reactivity
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Dog, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bat, Guinea pig, Pig, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
EIF3I / EIF3S2 antibody was raised against synthetic peptide located between aa128-177 of human EIF3I (Q13347, NP_003748). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Dog, Rabbit, Horse (100%); Mouse, Rat, Elephant, Panda, Bat, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (92%); Bovine, Platypus, Xenopus (85%).
Epitope
aa128-177
Specificity
Human EIF3I / EIF3S2
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
100 mM sodium phosphate, pH 7.6, 150 mM NaCl
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About EIF3I / EIF3S2
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. Q13347 NM_003757 NP_003748.1


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Images

Western blot

EIF3I / EIF3S2 antibody Western Blot of HeLa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3I / EIF3S2 antibody Western Blot of HeLa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EIF3I / EIF3S2 antibody Western Blot of HeLa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3I / EIF3S2 antibody Western Blot of HeLa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EIF3I / EIF3S2 antibody Western Blot of HeLa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3I / EIF3S2 antibody Western Blot of HeLa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EIF3I / EIF3S2 antibody Western Blot of HeLa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3I / EIF3S2 antibody Western Blot of HeLa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/15/2018

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