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Catalog Number Size Price
LS-C745313-25 25 µl (0.8 mg/ml) $275 
EIF3E Antibody - Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2). Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used.
EIF3E Antibody - Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).  The identity of the higher and lower molecular weight bands is unknown.  The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6.  Primary antibody was used at 1:1000.
EIF3E Antibody - Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2). Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used.
EIF3E Antibody - Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).  The identity of the higher and lower molecular weight bands is unknown.  The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6.  Primary antibody was used at 1:1000.
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Polyclonal Rabbit anti‑Mouse EIF3E Antibody (C‑Terminus, WB) LS‑C745313

Polyclonal Rabbit anti‑Mouse EIF3E Antibody (C‑Terminus, WB) LS‑C745313

Antibody:
EIF3E Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Mouse, Human, Monkey, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745313-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
EIF3E Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Mouse, Human, Monkey, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
EIF3E antibody LS-C745313 is an unconjugated rabbit polyclonal antibody to EIF3E (C-Terminus) from mouse. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
Target
Mouse EIF3E
Synonyms
EIF3E | EIF3-p46 | INT6 | EIF-3 p48 | EIF3-P48 | EIF3S6
Host
Rabbit
Reactivity
Mouse, Human, Monkey, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Predicted
Human, Monkey, Rat, Bovine, Dog (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-Terminus of mouse EIF3S6/Int6.
Epitope
C-Terminus
Specificity
This antibody is specific for mouse eIF3S6/Int6 protein. A BLAST analysis was used to suggest cross-reactivity with most eIF3Se/Int6 isoforms from mouse, human, rat, dog, bovine, and monkey based on 100% homology with the immunizing sequence. Cross-reactivity with EIF3S6/Int6 from other sources has not been determined.
Applications
  • Western blot (1:1000)
  • ELISA (1:80000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EIF3E
P60228 NM_001568 NP_001559.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/10/2021