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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF2S1 Antibody (phospho‑Ser51) LS‑C750565
EIF2S1 antibody LS-C750565 is an unconjugated rabbit polyclonal antibody to EIF2S1 from human, mouse and rat. Validated for IP and WB.
Catalog
Size
Price
LS-C750565-50
50 µl
$265
Description
EIF2S1 antibody LS-C750565 is an unconjugated rabbit polyclonal antibody to EIF2S1 from human, mouse and rat. Validated for IP and WB.
Target
Human EIF2S1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Epitope
pSer51
Usage
The predicted MW is 36kDa, while the observed MW by Western blot was 38kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About EIF2S1
P05198 NM_004094 NP_004085.1

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Immunoprecipitation

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb. Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb. Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.

Immunoprecipitation

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb. Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb. Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.

Immunoprecipitation

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb. Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb. Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.

Immunoprecipitation

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb. Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb. Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.

Requested From: United States
Date Requested: 11/18/2018
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