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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF2S1 Antibody (clone AT5E10) IHC‑plus™ LS‑B6273
Note: This antibody replaces LS-C139561
EIF2S1 antibody LS-B6273 is an unconjugated mouse monoclonal antibody to human EIF2S1. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6273-50
50 µl (1 mg/ml)
$425
Description
EIF2S1 antibody LS-B6273 is an unconjugated mouse monoclonal antibody to human EIF2S1. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EIF2S1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [AT5E10]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
EIF2S1 antibody was raised against recombinant protein
Specificity
Human EIF2S1
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About EIF2S1
P05198 NM_004094 NP_004085.1

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Images

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Extracts of HeLa (50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Extracts of HeLa (50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Extracts of HeLa (50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Extracts of HeLa (50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Extracts of HeLa (50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Extracts of HeLa (50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Extracts of HeLa (50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Extracts of HeLa (50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From: United States
Date Requested: 11/19/2018
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