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Antibodies > EIF2B3 > LS-C757914
 
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Catalog Number Size Price
LS-C757914-200 200 µl $393 
EIF2B3 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling EI2BG with EI2BG Antibody at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Mouse IgG (174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
EIF2B3 Antibody - All lanes: Anti-EI2BG Antibody at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
EIF2B3 Antibody - Overlay histogram showing U-2 OS cells stained with EI2BG Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (EI2BG Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (NH174309) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2b (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
EIF2B3 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling EI2BG with EI2BG Antibody at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Mouse IgG (174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
EIF2B3 Antibody - All lanes: Anti-EI2BG Antibody at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
EIF2B3 Antibody - Overlay histogram showing U-2 OS cells stained with EI2BG Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (EI2BG Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (NH174309) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2b (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Monoclonal Mouse anti‑Human EIF2B3 Antibody (clone 1778CT889.66.6.54, IF, WB) LS‑C757914

Monoclonal Mouse anti‑Human EIF2B3 Antibody (clone 1778CT889.66.6.54, IF, WB) LS‑C757914

Submit Review LSBio Guarantee
Antibody:
EIF2B3 Mouse anti-Human Monoclonal (1778CT889.66.6.54) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757914-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
EIF2B3 Mouse anti-Human Monoclonal (1778CT889.66.6.54) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EIF2B3 antibody LS-C757914 is an unconjugated mouse monoclonal antibody to human EIF2B3. Validated for ELISA, Flow, IF and WB.
Target
Human EIF2B3
Synonyms
EIF2B3 | EIF2Bgamma | EIF-2B
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1778CT889.66.6.54
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
This EI2BG antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 110-452 amino acids from human EI2BG.
Applications
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Usage
Western Blot Predicted Molecular Weight: 50.2 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EIF2B3
Q9NR50 NM_020365 NP_065098.1

Publications (0)

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Tag: GST
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See all EIF2B3 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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