PKR antibody LS-C17477 is an unconjugated rabbit polyclonal antibody to human PKR (EIF2AK2). Validated for ELISA, ICC, IF, IP and WB.
Human EIF2AK2 / PKR
(tested or 100% immunogen sequence identity)
- Western blot (1:1000)
- Immunoprecipitation (1:100)
EIF2AK2 / PKR antibody was raised against synthetic peptide correspoP9103-74Bnding to residues surrounding Thr451 of human PKR (KLH coupled).
Detects endogenous levels of human PKR at 74kD. Does not cross-react with other related proteins.
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry (for Immunofluorescence). Western Blot: 1:1000; incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100. Immunocytochemistry (for IF): 1:50.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.