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Catalog Number Size Price
LS-C808474-100 100 µl (1 mg/ml) $379 
LS-C808474-200 200 µl (1 mg/ml) $421 
EIF2AK2 / PKR Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
EIF2AK2 / PKR Antibody - Western blot analysis of PKR phosphorylation expression in Starvation treated K562 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
EIF2AK2 / PKR Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
EIF2AK2 / PKR Antibody - Western blot analysis of PKR phosphorylation expression in Starvation treated K562 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human EIF2AK2 / PKR Antibody (phospho‑Thr446, IHC, IF, WB) LS‑C808474

Polyclonal Rabbit anti‑Human EIF2AK2 / PKR Antibody (phospho‑Thr446, IHC, IF, WB) LS‑C808474

Antibody:
EIF2AK2 / PKR Rabbit anti-Human Polyclonal (pThr446) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C808474-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
EIF2AK2 / PKR Rabbit anti-Human Polyclonal (pThr446) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PKR antibody LS-C808474 is an unconjugated rabbit polyclonal antibody to human PKR (EIF2AK2) (pThr446). Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human EIF2AK2 / PKR
Synonyms
EIF2AK2 | p1/eIF-2A protein kinase | p68 kinase | PRKR | EIF-2A protein kinase 2 | p68 | Protein kinase RNA-activated | Tik
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PKR around the phosphorylation site of Threonine 446.
Epitope
pThr446
Specificity
Phospho-PKR (Thr446) Antibody detects endogenous levels of PKR only when phosphorylated at Threonine 446.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EIF2AK2 / PKR
P19525 NM_002759 NP_002750.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2024