Human EGFR

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse, Rat

IgG1 Monoclonal

H9B4

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Purified EGFR from A431 cells.

Human EGFR

• Western blot (0.5 - 1 µg/ml)
• Immunoprecipitation
• ELISA

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Epidermal Growth Factor Receptor is a type I receptor tyrosine kinase, referred to as EGFR, ErbB1 and HER1. When EGFR is activated by one of its ligands, it dimerizes. It can form a homodimer, heterodimers with other ErbB family members, or even a cluster of EGFRs. Activation stimulates EGFRs intracellular kinase activity. Autophosphorylation of tyrosine residues in the C-terminal domain of EGFR leads to association with proteins with phosphotyrosine-binding domains which then signal the initiation of signal transduction cascades including the JNK, MAPK, AKT, and possibly Nf-KB, pathways. Overexpression of EGFR is the cause of some types of cancer, including lung and colon cancer. It has also been linked to psoriasis, eczema and atherosclerosis, although poorly defined. Monoclonal antibody to EGFR can be used to block the extracellular ligand binding domain, therebye blocking tyrosine kinase activation and subsequent signal transduction. ELISA: Order BSA-free antibody format for coating.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P00533 NCBI: NM_005228 NP_005219.2