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Catalog Number Size Price
LS-C368940-30 30 µl (1 mg/ml) $253 
LS-C368940-100 100 µl (1 mg/ml) $305 
LS-C368940-200 200 µl (1 mg/ml) $369 
EGFR Antibody - Immunohistochemical analysis of EGFR (pY1016) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EGFR Antibody - Immunofluorescent analysis of EGFR (pY1016) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
EGFR Antibody - Western blot analysis of EGFR (pY1016) expression in A431 EGF-treated (A); Jurkat EGF-treated (B) whole cell lysates.
EGFR Antibody - Immunohistochemical analysis of EGFR (pY1016) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EGFR Antibody - Immunofluorescent analysis of EGFR (pY1016) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
EGFR Antibody - Western blot analysis of EGFR (pY1016) expression in A431 EGF-treated (A); Jurkat EGF-treated (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human EGFR Antibody (C‑Terminus, IHC, IF, WB) LS‑C368940

Polyclonal Rabbit anti‑Human EGFR Antibody (C‑Terminus, IHC, IF, WB) LS‑C368940

Antibody:
EGFR Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$253
LS-C368940-30
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
EGFR Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EGFR antibody LS-C368940 is an unconjugated rabbit polyclonal antibody to human EGFR (C-Terminus). Validated for ICC, IF, IHC, IP and WB.
Target
Human EGFR
Synonyms
EGFR | EGF Receptor | ERBB | ERBB1 | MENA | PIG61 | Proto-oncogene c-ErbB-1 | V-erb-b homolog | EGF-R | HER1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human EGFR.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of EGFR (pY1016) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EGFR
P00533 NM_005228 NP_005219.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2021