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Catalog Number Size Price
LS-C42950-0.1 0.1 mg (1 mg/ml) $525 
EGF Antibody - Staining of formalin-fixed, paraffin-embedded normal human skin with Rabbit anti-Human EGF (RABBIT ANTI HUMAN EGF).
EGF Antibody - Staining of formalin-fixed, paraffin-embedded normal human skin with Rabbit anti-Human EGF (RABBIT ANTI HUMAN EGF).
EGF Antibody - Staining of formalin-fixed, paraffin-embedded normal human skin with Rabbit anti-Human EGF (RABBIT ANTI HUMAN EGF).
EGF Antibody - Staining of formalin-fixed, paraffin-embedded normal human skin with Rabbit anti-Human EGF (RABBIT ANTI HUMAN EGF).
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Polyclonal Rabbit anti‑Human EGF Antibody (Extracellular Domain, IHC, WB) LS‑C42950

Polyclonal Rabbit anti‑Human EGF Antibody (Extracellular Domain, IHC, WB) LS‑C42950

Antibody:
EGF Rabbit anti-Human Polyclonal (Extracellular Domain) Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$525
LS-C42950-0.1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
EGF Rabbit anti-Human Polyclonal (Extracellular Domain) Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EGF antibody LS-C42950 is an unconjugated rabbit polyclonal antibody to human EGF (Extracellular Domain). Validated for ELISA, IHC and WB.
Target
Human EGF
Synonyms
EGF | Beta-urogastrone | HOMG4 | URG | Epidermal growth factor | Pro-epidermal growth factor
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Recombinant human EGF.
Epitope
Extracellular Domain
Specificity
Recognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, expressed by a variety of cells. The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the ~170 kD EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a (transforming growth factor alpha), VGF (vaccinia virus growth factor) and Betacellulin, expressed in the pancreas, small intestine and by certain tumor cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.
Applications
  • IHC - Paraffin (0.25 - 1 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin sections e. g. trypsin or pronase. Histology Positive Control Tissue: Human skin ELISA: LS-C42950 may be used in an indirect ELISA system or as a capture antibody in a sandwich ELISA for human EGF in combination with LS-C42951 as a detection reagent.
Presentation
PBS
Reconstitution
Distilled Water. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add azide.
Storage
Before reconstitution store at 4°C. After reconstitution store at -20°C. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EGF
P01133 NM_001963 NP_001954.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/27/2021