EDEM1 / EDEM antibody was raised against synthetic peptide located between aa146-195 of human EDEM1 (Q92611, NP_055489). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Hamster, Bovine, Rabbit, Pig, Guinea pig (100%); Rat, Dog, Chicken, Xenopus (92%); Opossum, Lizard (85%).
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
This antibody carries the LSBio 100% Guarantee.