EDEM antibody LS-C80983 is an unconjugated rabbit polyclonal antibody to EDEM (EDEM1) from human, mouse, bovine and other species. Validated for WB.
Human EDEM1 / EDEM
Human, Monkey, Mouse, Bovine, Guinea pig, Hamster, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot
- (applications tested for the base form of this product only)
EDEM1 / EDEM antibody was raised against synthetic peptide located between aa146-195 of human EDEM1 (Q92611, NP_055489). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Hamster, Bovine, Rabbit, Pig, Guinea pig (100%); Rat, Dog, Chicken, Xenopus (92%); Opossum, Lizard (85%).
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.