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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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E2F4 Antibody LS-C14983

Antibody

E2F4 Antibody LS-C14983

Antibody:
Mouse Monoclonal to Human E2F4
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$635 
LS-C14983-200
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human E2F4
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
E2F4 antibody LS-C14983 is an unconjugated mouse monoclonal antibody to E2F4 from human, mouse and rat. Validated for ELISA, IP and WB.

Target
Human E2F4
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • ELISA
  • (applications tested for the base form of this product only)
Immunogen
E2F4 antibody was raised against fusion Protein corresponding to the human E2F-4 protein.
Specificity
Recognizes E2F-4 at 55kD. Species cross-reactivity: Human, mouse and rat.
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml detected E2F4 in RIPA lysates from non-stimulated A431 cell lysate. Previous lots have detected E2F4 in HeLa nuclear extract, mouse 3T3 fibroblasts, and rat L6 skeletal myoblasts. Immunoprecipitation: 4-10 ug immunoprecipitated E2F4 from 500 ug of mouse 3T3/A431 cell lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About E2F4
Q16254 NM_001950 NP_001941.2

Publications (0)

Reviews (0)

Featured Products

Product
Species: Virus
Applications: Neutralization
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence
Reactivity: Human
Range: 0.2-50 ng/ml
Reactivity: Human
Range: 31.25-2000 pg/ml
Reactivity: Chicken
Range: 0.16-10 ng/ml

Requested From: United States
Date Requested: 7/15/2019
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