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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C14983-200 200 µg $635 
Antibody

Monoclonal Mouse anti‑Human E2F4 Antibody (WB) LS‑C14983

Monoclonal Mouse anti‑Human E2F4 Antibody (WB) LS‑C14983

Antibody:
Mouse Monoclonal to Human E2F4
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$635 
LS-C14983-200
200 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human E2F4
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
E2F4 antibody LS-C14983 is an unconjugated mouse monoclonal antibody to E2F4 from human. It is reactive with human, mouse and rat. Validated for ELISA, IP and WB.
Target
Human E2F4
Synonyms
E2F4 | E2F-4 | p107/p130-binding protein | Transcription factor E2F4
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • ELISA
Immunogen
Fusion Protein corresponding to the human E2F-4 protein.
Specificity
Recognizes E2F-4 at 55kD. Species cross-reactivity: Human, mouse and rat.
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml detected E2F4 in RIPA lysates from non-stimulated A431 cell lysate. Previous lots have detected E2F4 in HeLa nuclear extract, mouse 3T3 fibroblasts, and rat L6 skeletal myoblasts. Immunoprecipitation: 4-10 ug immunoprecipitated E2F4 from 500 ug of mouse 3T3/A431 cell lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About E2F4
Q16254 NM_001950 NP_001941.2

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Requested From: United States
Date Requested: 7/13/2020