E2F4 antibody LS-C14983 is an unconjugated mouse monoclonal antibody to E2F4 from human, mouse and rat. Validated for ELISA, IP and WB.
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
E2F4 antibody was raised against fusion Protein corresponding to the human E2F-4 protein.
Recognizes E2F-4 at 55kD. Species cross-reactivity: Human, mouse and rat.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml detected E2F4 in RIPA lysates from non-stimulated A431 cell lysate. Previous lots have detected E2F4 in HeLa nuclear extract, mouse 3T3 fibroblasts, and rat L6 skeletal myoblasts. Immunoprecipitation: 4-10 ug immunoprecipitated E2F4 from 500 ug of mouse 3T3/A431 cell lysate.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.