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Antibodies > DYKDDDDK Tag > LS-C742067
 
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Catalog Number Size Price
LS-C742067-40 40 µg $385 
DYKDDDDK Tag Antibody - Sensitivity and specificity of MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit to DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1: N-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2: N-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3: N-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4: N-terminal DYKDDDDK-tagged fusion protein (1 ng) Lane 5: C-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 6: C-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 7: C-terminal DYKDDDDK-tagged fusion protein Lane 8: C-terminal DYKDDDDK-tagged fusion protein (1 ng) Lane 9: Internal positions DYKDDDDK-tagged fusion protein (50 ng) (25 ng) Lane 11: Internal positions DYKDDDDK-tagged fusion protein (10 ng) Lane 12: Internal positions DYKDDDDK-tagged fusion protein (5 ng) Lane 13: Internal positions DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit 0.2 µg/ml Secondary Antibody: Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor,926-32211) (Licor,926-32211)
DYKDDDDK Tag Antibody - Western blot analysis of immunoprecipitation from cell lysates containing DYKDDDDK fusion protein by using MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit. Loading: Lane 1: MonoRabTM DYKDDDDK antibody (4 µg) + Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein Lane 2: Rabbit IgG (4 µg)++ Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein Lane 3: N-terminal DYKDDDDK tagged fusion protein (Input) Lane 4: MonoRabTM DYKDDDDK antibody (4 µg) + Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein Lane 5: Rabbit IgG (4 µg)++ Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein Lane 6: C-terminal DYKDDDDK tagged fusion protein (Input) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit 0.2 µg/ml Secondary Antibody: Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor, 926-32211)
DYKDDDDK Tag Antibody - MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit binds with DYKDDDDK Tag. Coating antigen: DYKDDDDK-tagged fusion protein, 1 µg/ml. MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit dilution start from 1,000 ng/ml. EC50= 47.78 ng/ml.
DYKDDDDK Tag Antibody - Sensitivity and specificity of MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit to DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1: N-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2: N-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3: N-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4: N-terminal DYKDDDDK-tagged fusion protein (1 ng) Lane 5: C-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 6: C-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 7: C-terminal DYKDDDDK-tagged fusion protein Lane 8: C-terminal DYKDDDDK-tagged fusion protein (1 ng) Lane 9: Internal positions DYKDDDDK-tagged fusion protein (50 ng) (25 ng) Lane 11: Internal positions DYKDDDDK-tagged fusion protein (10 ng) Lane 12: Internal positions DYKDDDDK-tagged fusion protein (5 ng) Lane 13: Internal positions DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit 0.2 µg/ml Secondary Antibody: Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor,926-32211) (Licor,926-32211)
DYKDDDDK Tag Antibody - Western blot analysis of immunoprecipitation from cell lysates containing DYKDDDDK fusion protein by using MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit. Loading: Lane 1: MonoRabTM DYKDDDDK antibody (4 µg) + Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein Lane 2: Rabbit IgG (4 µg)++ Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein Lane 3: N-terminal DYKDDDDK tagged fusion protein (Input) Lane 4: MonoRabTM DYKDDDDK antibody (4 µg) + Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein Lane 5: Rabbit IgG (4 µg)++ Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein Lane 6: C-terminal DYKDDDDK tagged fusion protein (Input) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit 0.2 µg/ml Secondary Antibody: Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor, 926-32211)
DYKDDDDK Tag Antibody - MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit binds with DYKDDDDK Tag. Coating antigen: DYKDDDDK-tagged fusion protein, 1 µg/ml. MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit dilution start from 1,000 ng/ml. EC50= 47.78 ng/ml.
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Monoclonal Rabbit DYKDDDDK Tag Antibody (clone 114F12C8, WB) LS‑C742067

Monoclonal Rabbit DYKDDDDK Tag Antibody (clone 114F12C8, WB) LS‑C742067

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Antibody:
DYKDDDDK Tag Rabbit Monoclonal (114F12C8) Antibody
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$385
LS-C742067-40
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Rabbit Monoclonal (114F12C8) Antibody
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C742067 is an unconjugated rabbit monoclonal antibody to DYKDDDDK Tag. Validated for ELISA, IP and WB.
Target
DYKDDDDK Tag
Host
Rabbit
Clonality
IgG Monoclonal
Clone
114F12C8
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • ELISA
Presentation
Lyophilized from PBS, pH 7.4, 0.02% Sodium Azide
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Storage
Long term: store at -20°C for up to 2 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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