DPYSL3 / CRMP4 antibody was raised against synthetic peptide corresponding to amino acids 499 to 511 of TUC-4a and amino acids 612-624 of TUC-4b (YDGPVFDLTTTPK). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Horse, Rabbit, Pig, Opossum, Platypus, Lizard (100%); Hamster, Bovine, Turkey, Chicken (92%).
Recognizes human TUC-4 protein (ULIP1/CRMP4). Reported to label early postmitotic neurons in prenatal rat brain. Not reactive with the proteins TUC-1 (ULIP3/CRMP1), TUC-2 (ULIP2/CRMP2) or TUC-3 (ULIP4/CRMP3). Does not label neural progenitor or radial glia. Species cross-reactivity: rat, mouse, feline and monkey.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:2500-1:25000 using alkaline phosphatase. Immunohistochemistry: 1:1000-1:5000 using PFA (DAB detection). Immunocytochemistry: 1:1000-1:5000 (DAB detection).
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
This antibody carries the LSBio 100% Guarantee.