CRMP4 antibody LS-C18222 is an unconjugated rabbit polyclonal antibody to CRMP4 (DPYSL3) from human, mouse, rat and other species. Validated for ICC, IHC, IP and WB.
Human DPYSL3 / CRMP4
Human, Monkey, Mouse, Rat, Dog, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
- IHC (1:1000 - 1:5000)
- ICC (1:1000 - 1:5000)
- Western blot (1:2500 - 1:25000)
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DPYSL3 / CRMP4 antibody was raised against synthetic peptide corresponding to amino acids 499 to 511 of TUC-4a and amino acids 612-624 of TUC-4b (YDGPVFDLTTTPK). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Horse, Rabbit, Pig, Opossum, Platypus, Lizard (100%); Hamster, Bovine, Turkey, Chicken (92%).
Recognizes human TUC-4 protein (ULIP1/CRMP4). Reported to label early postmitotic neurons in prenatal rat brain. Not reactive with the proteins TUC-1 (ULIP3/CRMP1), TUC-2 (ULIP2/CRMP2) or TUC-3 (ULIP4/CRMP3). Does not label neural progenitor or radial glia. Species cross-reactivity: rat, mouse, feline and monkey.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:2500-1:25000 using alkaline phosphatase. Immunohistochemistry: 1:1000-1:5000 using PFA (DAB detection). Immunocytochemistry: 1:1000-1:5000 (DAB detection).
0.2% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.