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Catalog Number Size Price
LS-C287786-10 10 µl $295 
LS-C287786-100 100 µl (0.2 mg/ml) $514 
DOT1L / DOT1 Antibody - Detection of Human DOT1L by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1 Detection: DAB.

Polyclonal Rabbit anti‑Human DOT1L / DOT1 Antibody (aa1000‑1050, IHC, WB) LS‑C287786

Polyclonal Rabbit anti‑Human DOT1L / DOT1 Antibody (aa1000‑1050, IHC, WB) LS‑C287786

Antibody:
DOT1L / DOT1 Rabbit anti-Human Polyclonal (aa1000-1050) Antibody
Application:
IHC, WB, IP, ChIP-Seq
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287786-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DOT1L / DOT1 Rabbit anti-Human Polyclonal (aa1000-1050) Antibody
Application:
IHC, WB, IP, ChIP-Seq
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DOT1 antibody LS-C287786 is an unconjugated rabbit polyclonal antibody to human DOT1 (DOT1L) (aa1000-1050). Validated for ChIP-Seq, IHC, IP and WB.
Target
Human DOT1L / DOT1
Synonyms
DOT1L | DOT1 | DOT1-like protein | KMT4 | Lysine N-methyltransferase 4 | KIAA1814 | H3-K79-HMTase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 84444 corresponding to amino acids between 1000 and 1050 of UniprotID: Q8TEK3
Epitope
aa1000-1050
Specificity
Region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 5 µg/mg lysate)
  • ChIP-sequencing
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. ChIP-Seq: 4 µg/30 µg chromatin
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DOT1L / DOT1
Q8TEK3 AK055359 NP_115871.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024