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Catalog Number Size Price
LS-C60846-1 1 mg (1 mg/ml) $405 
Mouse IgG Antibody - Western Blot of Biotin Donkey Anti-Mouse IgG Pre-Adsorbed secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin donkey secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.

Polyclonal Donkey Anti‑Mouse IgG Secondary Antibody (Biotin, IHC, WB) LS‑C60846

Polyclonal Donkey Anti‑Mouse IgG Secondary Antibody (Biotin, IHC, WB) LS‑C60846

Antibody:
Mouse IgG Donkey anti-Mouse Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA, IE
Reactivity:
Mouse
Format:
Biotin, Unmodified
Price
Catalog Number
$405
LS-C60846-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Mouse IgG Donkey anti-Mouse Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA, IE
Reactivity:
Mouse
Format:
Biotin, Unmodified

Specifications

Description
Mouse IgG antibody LS-C60846 is a biotin-conjugated donkey polyclonal antibody. Validated for ELISA, IE, IHC and WB.
Host
Donkey
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
  • Immunoelectrophoresis
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024