Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit (tested or 100% immunogen sequence identity)
Specificity and Use
Rabbit IgG antibody was raised against rabbit IgG whole molecule.
anti-Donkey Serum, Rabbit IgG, and Rabbit Serum
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide.
For research use only.
About Rabbit IgG
IgG is the most common type of antibody found in the circulation, representing approximately 75% of serum antibodies. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape.
Dot Blot showing the detection of Rabbit IgG. A three-fold serial dilution of Rabbit IgG starting at 200ng was spotted onto 0.45 um nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20C, Anti-Rabbit IgG (H&L) (DONKEY) Antibody Texas Red Conjugated (p/n LS-C60953) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP. This image was taken for the unconjugated form of this product. Other forms have not been tested.