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Catalog Number Size Price
LS-C19023-100 100 µg $567 
DNMT3L Antibody - Immunohistochemistry of Rabbit Anti-Human DNMT3L Antibody at 1:500.
DNMT3L Antibody - Anti-DNMT3L Antibody - Western Blot. Affinity Purified Rabbit anti-DNMT3L was used at a 1:1500 dilution to detect human DNMT3L by western blot after immunoprecipitation using the same antibody. Transfection of U2OS cells (10cm dish) was accomplished using 3 ug of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For IP and WB conditions see Fuks et al. (2000). For western blotting the antibody was used at 0.4 ug/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL. Note: this antibody works for western blotting only following immunoprecipitation. We have not had good success using the antibody directly for western blotting. The expected molecular weight of human DNMT3L is 43.6 kD.
DNMT3L Antibody - Immunohistochemistry of Rabbit Anti-Human DNMT3L Antibody at 1:500.
DNMT3L Antibody - Anti-DNMT3L Antibody - Western Blot. Affinity Purified Rabbit anti-DNMT3L was used at a 1:1500 dilution to detect human DNMT3L by western blot after immunoprecipitation using the same antibody. Transfection of U2OS cells (10cm dish) was accomplished using 3 ug of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For IP and WB conditions see Fuks et al. (2000). For western blotting the antibody was used at 0.4 ug/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL. Note: this antibody works for western blotting only following immunoprecipitation. We have not had good success using the antibody directly for western blotting. The expected molecular weight of human DNMT3L is 43.6 kD.
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Polyclonal Rabbit anti‑Human DNMT3L Antibody (IHC, WB) LS‑C19023

Polyclonal Rabbit anti‑Human DNMT3L Antibody (IHC, WB) LS‑C19023

Note: This antibody replaces LS-C64796
Antibody:
DNMT3L Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C19023-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DNMT3L Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DNMT3L antibody LS-C19023 is an unconjugated rabbit polyclonal antibody to human DNMT3L. Validated for ELISA, IHC, IP and WB.
Target
Human DNMT3L
Synonyms
DNMT3L
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.
Specificity
This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. Reactivity with DNMT3L from other species has not yet been tested.
Applications
  • IHC (1:500)
  • Western blot (1:500)
  • Immunoprecipitation (1:100)
  • ELISA (1:1000 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DNMT3L
Q9UJW3 NM_013369 NP_037501.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024