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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DLL4 Antibody (Internal) LS-C19035

Best Seller
Note: This antibody replaces LS-C64649
DLL4 antibody LS-C19035 is an unconjugated rabbit polyclonal antibody to human DLL4. Validated for ELISA, ICC, IF, IHC and WB. Cited in 2 publications.
Catalog
Size
Price
LS-C19035-100
100 µg (1 mg/ml)
$425
Description
DLL4 antibody LS-C19035 is an unconjugated rabbit polyclonal antibody to human DLL4. Validated for ELISA, ICC, IF, IHC and WB. Cited in 2 publications.
Target
Human DLL4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:2500)
  • ELISA (1:5000 - 1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
DLL4 antibody was raised against synthetic peptide from human DLL4.
Epitope
Internal
Specificity
An internal region of Human DELTA-4.
Usage
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 74 kD in size corresponding to DELTA-4 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About DLL4
Q9NR61 NM_019074 NP_061947.1

Popular DLL4 Products

IHC of paraffin-embedded rectum cancer tissues using DLL4 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IHC with DLL4 antibody on frozen human tonsil.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Dog
Applications: IHC, IHC - Frozen, Western blot
Antibody
Species: Mouse
Applications: ELISA
Western blot of DLL4 in HUVEC cell lysate using 1) preimmune sera at 1:5000 and 2) Polyclonal Antibody to DLL4 at 3 ug/ml, and 3) mouse embryo body lysate at 6 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Species: Human, Mouse
Applications: Western blot

Publications (2)

1
Anthrax toxins cooperatively inhibit endocytic recycling by the Rab11/Sec15 exocyst. Guichard A, McGillivray SM, Cruz-Moreno B, van Sorge NM, Nizet V, Bier E. Nature. 2010 467:854-8. (ICC; Human) [PubMed:20944747]
2
Cholera toxin disrupts barrier function by inhibiting exocyst-mediated trafficking of host proteins to intestinal cell junctions. Guichard A, Cruz-Moreno B, Cruz-Moreno BC, Aguilar B, van Sorge NM, Kuang J, Kurkciyan AA, Wang Z, Hang S, Pineton de Chambrun GP, McCole DF, Watnick P, Nizet V, Bier E. Cell host & microbe. 2013 Sep;14:294-305. [Full Text Article] [PubMed:24034615] [PMC:PMC3786442]

Customer Reviews (0)


Images

Immunohistochemistry

Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Western blot

Western Blot - Anti-Delta-4 Antibody. Western blot of Affinity Purified anti-Delta-4 antibody shows detection of a 74-kD band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.
Western Blot - Anti-Delta-4 Antibody. Western blot of Affinity Purified anti-Delta-4 antibody shows detection of a 74-kD band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.

Immunohistochemistry

Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Western blot

Western Blot - Anti-Delta-4 Antibody. Western blot of Affinity Purified anti-Delta-4 antibody shows detection of a 74-kD band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.
Western Blot - Anti-Delta-4 Antibody. Western blot of Affinity Purified anti-Delta-4 antibody shows detection of a 74-kD band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.

Immunohistochemistry

Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Western blot

Western Blot - Anti-Delta-4 Antibody. Western blot of Affinity Purified anti-Delta-4 antibody shows detection of a 74-kD band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.
Western Blot - Anti-Delta-4 Antibody. Western blot of Affinity Purified anti-Delta-4 antibody shows detection of a 74-kD band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.

Immunohistochemistry

Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Western blot

Western Blot - Anti-Delta-4 Antibody. Western blot of Affinity Purified anti-Delta-4 antibody shows detection of a 74-kD band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.
Western Blot - Anti-Delta-4 Antibody. Western blot of Affinity Purified anti-Delta-4 antibody shows detection of a 74-kD band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.

Requested From: United States
Date Requested: 4/21/2019
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