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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DKK1 Antibody (Cytoplasmic Domain) IHC‑plus™ LS‑A2867 Best Seller
Note: This antibody replaces LS-C64745, LS-C42470
DKK1 antibody LS-A2867 is an unconjugated rabbit polyclonal antibody to human DKK1. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Catalog
Size
Price
LS-A2867-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment
Description
DKK1 antibody LS-A2867 is an unconjugated rabbit polyclonal antibody to human DKK1. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human DKK1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
DKK1 antibody was raised against synthetic 15 amino acid peptide from the cytoplasmic domain of human DKK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (93%); Marmoset (80%).
Epitope
Cytoplasmic Domain
Specificity
Human DKK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A2867 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A2867 was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About DKK1
O94907 NM_012242 NP_036374.1

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Western blot of human DKK1 in A549 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) RAW using antibody at 1 ug/ml.
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Publications (2)

1
Nitric oxide production upregulates Wnt/ beta -catenin signaling by inhibiting Dickkopf-1. Du Q, Zhang X, Liu Q, Zhang X, Bartels CE, Geller DA. Cancer research. 2013 Nov;73:6526-37. [Full Text Article] [PubMed:24008318] [PMC:PMC3818363]
2
Wnt antagonist DKK1 acts as a tumor suppressor gene that induces apoptosis and inhibits proliferation in human renal cell carcinoma. Hirata H, Hinoda Y, Nakajima K, Kawamoto K, Kikuno N, Ueno K, Yamamura S, Zaman MS, Khatri G, Chen Y, Saini S, Majid S, Deng G, Ishii N, Dahiya R. International journal of cancer. Journal international du cancer. 2011 Apr;128:1793-803. [Full Text Article] [PubMed:20549706]

Customer Reviews (0)


Images

Immunohistochemistry

Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 12/11/2018
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