DKK1 antibody LS-A2867 is an unconjugated rabbit polyclonal antibody to human DKK1. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
(tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Also available conjugated with AP
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000)
- (applications tested for the base form of this product only)
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DKK1 antibody was raised against synthetic 15 amino acid peptide from the cytoplasmic domain of human DKK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (93%); Marmoset (80%).
Human DKK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A2867 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A2867 was determined to be 10 ug/ml.
PBS, 0.1% Sodium Azide
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
For research use only.