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| Catalog Number | Size | Price |
|---|---|---|
| LS-C61730-100 | 100 µg (0.5 mg/ml) | $503 |
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Polyclonal Goat anti‑Human DIO2 Antibody (aa217‑228, IHC, IF) LS‑C61730
Polyclonal Goat anti‑Human DIO2 Antibody (aa217‑228, IHC, IF) LS‑C61730
Antibody:
DIO2 Goat anti-Human Polyclonal (aa217-228) Antibody
Application:
IHC, IF, Peptide-ELISA
Reactivity:
Human, Mouse, Pig
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
DIO2 Goat anti-Human Polyclonal (aa217-228) Antibody
Application:
IHC, IF, Peptide-ELISA
Reactivity:
Human, Mouse, Pig
Format:
Unconjugated, Unmodified
Specifications
Description
DIO2 antibody LS-C61730 is an unconjugated goat polyclonal antibody to DIO2 (aa217-228) from human. It is reactive with human, mouse and pig. Validated for IF, IHC and Peptide-ELISA.
Target
Human DIO2
Synonyms
DIO2 | 5DII | D2 | DIOII | ITDI2 | Type 2 DI | TXDI2 | Type-II 5-deiodinase | SelY | Type-II 5deiodinase | Thyroxine deiodinase, type II
Host
Goat
Reactivity
Human, Mouse, Pig
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence EVKKHQNQEDRC, from the internal region of the protein sequence according to NP_000784.2NP_054644.1NP_001007024.1.
Epitope
aa217-228
Specificity
Human DIO2. This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).
Applications
- IHC
- Immunofluorescence (10 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
IHC: Cryo-sectioned (4% PFA-fixed) Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration, 2-3 ug/ml. Data obtained from anonymous customer. Western blot: Preliminary experiments gave an approx 65-70 kDa band in Human Heart and Thyroid lysates after 0.05 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5 kDa according to NP_NP_000784.2 and of 34.6 kDa according to NP_001007024.1. The 65-70 kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About DIO2
Publications (0)
Customer Reviews (0)
Featured Products
Reactivity:
Mouse
Range:
0.39-25 ng/ml
Species:
Human
Applications:
Flow Cytometry, Functional Assay
Reactivity:
Monkey
Range:
15.63-1000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
