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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Digoxin Antibody (AP) LS-C507570

Digoxin antibody LS-C507570 is an AP-conjugated mouse monoclonal antibody to Digoxin. Validated for ELISA.
Catalog
Size
Price
LS-C507570-100
100 µl
$905
Description
Digoxin antibody LS-C507570 is an AP-conjugated mouse monoclonal antibody to Digoxin. Validated for ELISA.
Target
Digoxin
Host
Mouse
Clonality
IgG1 Monoclonal
Conjugations
Alkaline Phosphatase. Also available Unconjugated or conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • ELISA
  • (applications tested for the base form of this product only)
Immunogen
Digoxin antibody was raised against digoxin-BSA.
Specificity
Recognizes Digoxin.
Presentation
PBS, pH 7.2, No preservatives added
Storage
Store at 4°C.
Restrictions
For research use only.

Popular Digoxin Products

Antibody
Species:
Applications: Western blot, ELISA
Antibody
Species:
Applications: ELISA, Radioimmunoassay
Antibody
Species:
Applications: ELISA, Radioimmunoassay
Western blot analysis of Digoxin was performed by loading 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. BSA was detected at ~66kD using a BSA monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform. Digoxin-labeled BSA was detected at ~66kD using a Digoxin monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.
Species:
Applications: Western blot, Immunoprecipitation, ELISA, Radioimmunoassay
Western blot analysis of Digoxin was performed by loading 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. BSA was detected at ~66kD using a BSA monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform. Digoxin-labeled BSA was detected at ~66kD using a Digoxin monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.
Species:
Applications: Western blot, Immunoprecipitation, ELISA, Radioimmunoassay

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Requested From: United States
Date Requested: 3/19/2019
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