Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
206-374-1102
Fax:
206-577-4565
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C19007-100 100 µg (1.4 mg/ml) $498 
DIAPH2 Antibody - Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Polyclonal Rabbit anti‑Human DIAPH2 Antibody (C‑Terminus, WB) LS‑C19007

Polyclonal Rabbit anti‑Human DIAPH2 Antibody (C‑Terminus, WB) LS‑C19007

Note: This antibody replaces LS-C64718
Antibody:
DIAPH2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C19007-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DIAPH2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DIAPH2 antibody LS-C19007 is an unconjugated rabbit polyclonal antibody to human DIAPH2 (C-Terminus). Validated for ELISA, IP and WB. Cited in 1 publication.
Target
Human DIAPH2
Synonyms
DIAPH2 | DRF2 | DIA | DIA2 | POF | POF2 | Protein diaphanous homolog 2 | Diaphanous-related formin-2 | Diaphorase-2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.
Epitope
C-Terminus
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse DIA-2 based on a 93% homology to the immunogen sequence. Cross reactivity with DIA-2 homologues from other sources has not been determined.
Applications
  • Western blot (1:1000 - 1:6000)
  • Immunoprecipitation (1:100)
  • ELISA (1:12000 - 1:60000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kD in size corresponding to DIA-2 by western blotting in the appropriate cell lysate or extract. Alternate splice variants have been described for this protein. This antibody will only react with DIA-156 (isoform 1 of DIA-2).
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DIAPH2
O60879 NM_006729 NP_006720.1

LSBio Ratings

DIAPH2 Antibody (C-Terminus) for WB/Western, IP, ELISA LS-C19007 has an LSBio Rating of
Publications (4)

Publications (1)

Diaphanous formin mDia2 regulates CENP-A levels at centromeres. Liu C, Mao Y. The Journal of cell biology. 2016 213:415-24. (WB; Human)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022