DIAPH2 | DRF2 | DIA | DIA2 | POF | POF2 | Protein diaphanous homolog 2 | Diaphanous-related formin-2 | Diaphorase-2
(tested or 100% immunogen sequence identity)
Mouse (at least 90% immunogen sequence identity)
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.
A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse DIA-2 based on a 93% homology to the immunogen sequence. Cross reactivity with DIA-2 homologues from other sources has not been determined.
- Western blot (1:1000 - 1:6000)
- Immunoprecipitation (1:100)
- ELISA (1:12000 - 1:60000)
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kD in size corresponding to DIA-2 by western blotting in the appropriate cell lysate or extract. Alternate splice variants have been described for this protein. This antibody will only react with DIA-156 (isoform 1 of DIA-2).
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.