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Catalog Number Size Price
LS-C19007-100 100 µg (1.4 mg/ml) $567 
DIAPH2 Antibody - Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Polyclonal Rabbit anti‑Human DIAPH2 Antibody (C‑Terminus, WB) LS‑C19007

Polyclonal Rabbit anti‑Human DIAPH2 Antibody (C‑Terminus, WB) LS‑C19007

Note: This antibody replaces LS-C64718
Antibody:
DIAPH2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C19007-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DIAPH2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DIAPH2 antibody LS-C19007 is an unconjugated rabbit polyclonal antibody to human DIAPH2 (C-Terminus). Validated for ELISA, IP and WB. Cited in 1 publication.
Target
Human DIAPH2
Synonyms
DIAPH2 | DRF2 | DIA | DIA2 | POF | POF2 | Protein diaphanous homolog 2 | Diaphanous-related formin-2 | Diaphorase-2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.
Epitope
C-Terminus
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse DIA-2 based on a 93% homology to the immunogen sequence. Cross reactivity with DIA-2 homologues from other sources has not been determined.
Applications
  • Western blot (1:1000 - 1:6000)
  • Immunoprecipitation (1:100)
  • ELISA (1:12000 - 1:60000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kD in size corresponding to DIA-2 by western blotting in the appropriate cell lysate or extract. Alternate splice variants have been described for this protein. This antibody will only react with DIA-156 (isoform 1 of DIA-2).
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DIAPH2
O60879 NM_006729 NP_006720.1

LSBio Ratings

DIAPH2 Antibody (C-Terminus) for WB/Western, IP, ELISA LS-C19007 has an LSBio Rating of
Publications (4)

Publications (1)

Diaphanous formin mDia2 regulates CENP-A levels at centromeres. Liu C, Mao Y. The Journal of cell biology. 2016 213:415-24. (WB; Human)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024