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Catalog Number Size Price
LS-B14863-50 50 µl $460 
DHFR Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
DHFR Antibody - Immunohistochemistry of paraffin-embedded human liver injury tissue.
DHFR Antibody - Immunohistochemistry of paraffin-embedded mouse lung using DHFR antibody at dilution of 1:100 (200x lens).
DHFR Antibody - Immunohistochemistry of paraffin-embedded rat kidney using DHFR antibody at dilution of 1:100 (200x lens).
DHFR Antibody - Western blot analysis of extracts of various cell lines, using DHFR antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
DHFR Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
DHFR Antibody - Immunohistochemistry of paraffin-embedded human liver injury tissue.
DHFR Antibody - Immunohistochemistry of paraffin-embedded mouse lung using DHFR antibody at dilution of 1:100 (200x lens).
DHFR Antibody - Immunohistochemistry of paraffin-embedded rat kidney using DHFR antibody at dilution of 1:100 (200x lens).
DHFR Antibody - Western blot analysis of extracts of various cell lines, using DHFR antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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IHC‑plus™ Polyclonal Rabbit anti‑Human DHFR Antibody (IHC, IF, WB) LS‑B14863

IHC‑plus™ Polyclonal Rabbit anti‑Human DHFR Antibody (IHC, IF, WB) LS‑B14863

Note: This antibody replaces LS-C331576
Antibody:
DHFR Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B14863-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DHFR Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
DHFR antibody LS-B14863 is an unconjugated rabbit polyclonal antibody to DHFR from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human DHFR
Synonyms
DHFR | DHFRP1 | Dihydrofolate reductase | DYR
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human DHFR (NP_000782.1). MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
Specificity
Human DHFR
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 15kDa/21kDa, while the observed MW by Western blot was 19kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DHFR
P00374 NM_000791 NP_000782.1

Validation

DHFR Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ DHFR Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B14863 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024