Human DHAND / HAND2

Goat

Human, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

E. coli-derived rhHAND2.

Recognizes human HAND2. Species cross-reactivity: Mouse.

• IHC (10 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.5-1.0 ug/ml. The detection limit for rhHAND2 and rmHAND2 is approximately 1.3 ng/well. Western Blot: 0.1-0.2 ug/ml. The detection limit for rhHAND2 and rmHAND2 is approximately 25 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 10 ug/ml to detect HAND2 in mouse embryos. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at RT in the dark. Between each step, cells were washed with PBS containing BSA.

Lyophilized from Sterile Solution PBS, 5% Trehalose

Reconstitute with sterile 40-50% glycerol, PBS.

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P61296 NCBI: NM_021973 NP_068808.1