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Catalog Number Size Price
LS-B3186-0.05 0.05 mg (1 mg/ml) $460 
DFFA / ICAD / DFF45 Antibody - Anti-DFFA / ICAD antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
DFFA / ICAD / DFF45 Antibody - Anti-DFFA / ICAD antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
DFFA / ICAD / DFF45 Antibody - Anti-DFFA / ICAD antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
DFFA / ICAD / DFF45 Antibody - Western blot analysis of DFF45 in HeLa cell lysate with DFF45 antibody at (A) 1 and (B) 2 µg/mL.
DFFA / ICAD / DFF45 Antibody - Western blot of DFF45/35 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with anti-DFF45/35 (NT) at 1:1000 dilution.
DFFA / ICAD / DFF45 Antibody - Anti-DFFA / ICAD antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
DFFA / ICAD / DFF45 Antibody - Anti-DFFA / ICAD antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
DFFA / ICAD / DFF45 Antibody - Anti-DFFA / ICAD antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
DFFA / ICAD / DFF45 Antibody - Western blot analysis of DFF45 in HeLa cell lysate with DFF45 antibody at (A) 1 and (B) 2 µg/mL.
DFFA / ICAD / DFF45 Antibody - Western blot of DFF45/35 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with anti-DFF45/35 (NT) at 1:1000 dilution.
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IHC‑plus™ Polyclonal Rabbit anti‑Human DFFA / ICAD / DFF45 Antibody (IHC, WB) LS‑B3186

IHC‑plus™ Polyclonal Rabbit anti‑Human DFFA / ICAD / DFF45 Antibody (IHC, WB) LS‑B3186

Note: This antibody replaces LS-C269
Antibody:
DFFA / ICAD / DFF45 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B3186-0.05
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DFFA / ICAD / DFF45 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DFF45 antibody LS-B3186 is an unconjugated rabbit polyclonal antibody to human DFF45 (DFFA / ICAD). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human DFFA / ICAD / DFF45
Synonyms
DFFA | DFF-45 | DFF1 | Dna fragmentation factor-45 | ICAD | Inhibitor of CAD | DFF45
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
DEAE chromatography
Modifications
Unmodified
Immunogen
A 20 amino acid peptide near the N-Terminus of human DFF45. The immunogen is located within the first 50 amino acids of DFF45.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
DFF45 antibody can be used for detection of DFF45, DFF35, and one the cleaved fragment by Western blot at 1:1000 to 1:2000 dilution. 45 and 35 kDa bands can be detected in non-apoptotic cells. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot: 45 and 35 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DFFA / ICAD / DFF45
O00273 NM_004401 NP_004392.1

Validation

DFFA / ICAD / DFF45 Antibody - Anti-DFFA / ICAD antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-DFFA / ICAD antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-DFFA / ICAD antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

DFFA / ICAD / DFF45 Antibody - Anti-DFFA / ICAD antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-DFFA / ICAD antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-DFFA / ICAD antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

DFFA / ICAD / DFF45 Antibody - Anti-DFFA / ICAD antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-DFFA / ICAD antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-DFFA / ICAD antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ DFFA / ICAD / DFF45 Antibody for IHC, WB/Western, ELISA LS-B3186 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024