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DBT / E2 Antibody (aa35‑84) IHC‑plus™ LS‑B4677
Note: This antibody replaces LS-C111356
E2 antibody LS-B4677 is an unconjugated rabbit polyclonal antibody to E2 (DBT) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
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Species:
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot
Product Description
E2 antibody LS-B4677 is an unconjugated rabbit polyclonal antibody to E2 (DBT) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About DBT / E2
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2.
P11182
NM_001918
NP_001909.3
DBT Antibody, BCKAD E2 subunit Antibody, BCKAD-E2 Antibody, BCKADE2 Antibody, Dihydrolipoyl transacylase Antibody, E2 Antibody, E2B Antibody, BCATE2 Antibody
Specifications
Target
Human DBT / E2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
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Immunogen
DBT / E2 antibody was raised against synthetic peptide located between aa35-84 of human DBT (P11182, NP_001909). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Zebrafish, Beetle (92%); Trout, Drosophila (91%); Stickleback, Sea squirt (85%).
Blocking Peptide
LS-E18663 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4677. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-B4677. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa35-84
Specificity
Human DBT / E2
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
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Images
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
DBT / E2 antibody LS-B4677 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
DBT / E2 antibody LS-B4677 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
DBT / E2 antibody LS-B4677 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry
Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
DBT / E2 antibody LS-B4677 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From: United States
Date Requested: 9/23/2018
Date Requested: 9/23/2018
