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DBF4

DBF4 homolog (S. cerevisiae)

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

Gene Name: DBF4 homolog (S. cerevisiae)
Synonyms: DBF4, Activator of S phase kinase, CHIF, DBF4 homolog (S. cerevisiae), Chiffon homolog A, DBF4A, ZDBF1, Protein DBF4 homolog A, ASK
Target Sequences: NM_006716 NP_006707.1 Q9UBU7

☰ Filters
Antibodies (25)
Proteins (5)
Over-Expression Lysate (2)
Primary (25)
Recombinant (3)
DBF4 (30)
Human (23)
Mouse (10)
Bat (3)
Bovine (1)
Dog (3)
Gibbon (3)
Horse (3)
Monkey (4)
Pig (4)
Rabbit (3)
IHC (4)
IHC-P (2)
WB (22)
ELISA (5)
ICC (1)
IF (3)
Peptide-ELISA (3)
rabbit (22)
mouse (2)
goat (1)
IgG (15)
IgG1 (1)
IgG1,k (1)
monoclonal mc (2)
polyclonal pc (23)
6G9 (1)
APC Conjugated (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (14)
aa581-630 (4)
10-90 aa, N-terminal (1)
Ile18 (1)
N-Terminus (1)
aa10-59 (1)
aa585-599 (1)
No (30)
GST (2)
GST, N-terminus (1)
Myc-DDK (Flag) (2)
Human (4)
293T Cells (1)
HEK 293 Cells (1)
Wheat Germ Extract (3)
Purified (2)
DBF4 Antibody - Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell. [antibody concentration 10 ug/ml].
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DBF4 Mouse anti-Human Monoclonal (6G9) Antibody
Human
ELISA, IF, WB
Unconjugated
100 µg/$423
DBF4 Antibody - Western blot of Dbf4 antibody
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DBF4 Rabbit anti-Human Polyclonal (10-90 aa, N-terminal) Antibody
Mouse, Human, Monkey
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
DBF4 Antibody - DBF4 antibody Western Blot of Mouse Brain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DBF4 Rabbit anti-Mouse Polyclonal (aa581-630) Antibody
Mouse
WB
Unconjugated
100 µl/$424
DBF4 Antibody - DBF4 antibody Western Blot of Mouse Brain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DBF4 Rabbit anti-Mouse Polyclonal (aa581-630) (HRP) Antibody
Rabbit, Mouse, Dog, Pig, Horse, Bat, Gibbon, Human, Monkey
WB
HRP Conjugated
100 µl/$469
DBF4 Antibody - DBF4 antibody Western Blot of Mouse Brain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DBF4 Rabbit anti-Mouse Polyclonal (aa581-630) (Biotin) Antibody
Rabbit, Mouse, Dog, Pig, Horse, Bat, Gibbon, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
DBF4 Antibody - DBF4 antibody Western Blot of Mouse Brain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DBF4 Rabbit anti-Mouse Polyclonal (aa581-630) (FITC) Antibody
Rabbit, Mouse, Dog, Pig, Horse, Bat, Gibbon, Human, Monkey
WB
FITC Conjugated
100 µl/$469
DBF4 Antibody - Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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DBF4 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Bovine, Pig, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$299
DBF4 Protein - 12.5% SDS-PAGE of human DBF4 stained with Coomassie Blue
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Wheat Germ Extract
GST
99.66 kDa
2 µg/$439
DBF4 Antibody - Immunohistochemistry image of paraffin-embedded human testis tissue at a dilution of 1:100
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DBF4 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$294; 100 µg/$360
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DBF4 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
HRP Conjugated
50 µg/$294; 100 µg/$360
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DBF4 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
FITC Conjugated
50 µg/$294; 100 µg/$360
DBF4 Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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DBF4 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
DBF4 Antibody - Western blot of Dbf4 (I18) pAb in extracts from NIH-3T3 treated with H2O2 100uM 30'.
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DBF4 Rabbit anti-Human Polyclonal (Ile18) Antibody
Mouse, Human
IHC, WB
Unconjugated
50 µl/$321; 100 µl/$403
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DBF4 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$341
DBF4 Antibody - Western blot analysis of extracts from NIH-3T3 cells treated with H2O2 (100uM, 30mins) and COS-7 cells treated with PMA (125mg/ml, 30mins), using DBF4 antibody.
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DBF4 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, WB
Unconjugated
100 µg/$357
DBF4 Antibody - DBF4 antibody (1 ug/ml) staining of Mouse Testis lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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DBF4 Goat anti-Mouse Polyclonal (aa585-599) Antibody
Mouse
Peptide-ELISA, WB
Unconjugated
100 µg/$503
DBF4 Antibody - Western blot analysis of lysates from NIH/3T3 cells, treated with H2O2 100uM 30', COS7 treated with PMA 125ng/ml 30',using DBF4 Antibody. The lane on the right is blocked with the synthesized peptide.
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DBF4 Rabbit anti-Human Polyclonal (aa10-59) Antibody
Mouse, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
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DBF4 Mouse anti-Human Monoclonal Antibody
Human
WB
Unconjugated
100 µg/$786
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DBF4 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µg/$749
Select
DBF4 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
WB
HRP Conjugated
100 µl/$937
Select
DBF4 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$937
Select
DBF4 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
WB
FITC Conjugated
100 µl/$937
Select
DBF4 Rabbit anti-Human Polyclonal (PE) Antibody
Human
WB
PE Conjugated
100 µl/$937
Select
DBF4 Rabbit anti-Human Polyclonal (APC) Antibody
Human
WB
APC Conjugated
100 µl/$937
DBF4 Antibody - All lanes : Anti-DBF4 Antibody at 1:1000 dilution Lane 1: NIH/3T3 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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DBF4 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µl/$386
Viewing 1-25 of 30 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).