Cytokeratin AE1+AE3

Mouse

Human, Mouse, Rat, Rabbit, Chicken, Cow (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Unmodified

Human epithelial keratins

• IHC - Paraffin (1:20 - 1:50)
• IHC - Frozen (1:20 - 1:50)

Paraffin sections may be pre-treated with 1 % protease solution for 15 minutes at RT, improvement of staining may be achieved with antigen retrieval. Pan-Cytokeratin (AE1/AE3) contains two different clones, which label the majority of normal epithelia and carcinoma. Cytokeratins are a group of water soluble filament proteins, which are components of the cytoskeleton of the epidermis and other epithelial cells. Gelelectrophoretic investigation revealed 20 different Cytokeratins, characterised by different molecular sizes and pI-values. They can be subdivided into basic and acid subfamilies. The nomenclature according to Moll et al. (1982) is most commonly used. Basic cytokeratins (52-67 kD) and acid cytokeratins (40, 48, 50, 50', 56,5 kD) CK 1-8, 10, 14-16 and 19 according to the nomenclature by Moll (1982) .

PBS, pH 7.3, 0.09% Sodium Azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.