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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C391361-100 100 µg (1 mg/ml) $465 
Cytokeratin 10+13 Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with Cytokeratin 10 + 13 antibody (SPM262).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Cytokeratin 10+13 Antibody - Western blot testing of A431 cell lysate and Cytokeratin 10 + 13 antibody (clone SPM262). This image was taken for the unmodified form of this product. Other forms have not been tested.
Cytokeratin 10+13 Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with Cytokeratin 10 + 13 antibody (SPM262).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Cytokeratin 10+13 Antibody - Western blot testing of A431 cell lysate and Cytokeratin 10 + 13 antibody (clone SPM262). This image was taken for the unmodified form of this product. Other forms have not been tested.
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Cytokeratin 10+13 Antibody (clone SPM262, Azide‑free) LS‑C391361

Cytokeratin 10+13 Antibody (clone SPM262, Azide‑free) LS‑C391361

Antibody:
Mouse Monoclonal to Human Cytokeratin 10+13
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free
Other formats:
Price
Catalog Number
$465 
LS-C391361-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Cytokeratin 10+13
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free
Other formats:

Specifications

Description
Cytokeratin 10+13 antibody LS-C391361 is an unconjugated mouse monoclonal antibody to human Cytokeratin 10+13. Validated for Flow, IF, IHC and WB.
Target
Human Cytokeratin 10+13
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
SPM262
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Western blot (0.25 - 0.5 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium.
Specificity
Human Cytokeratin 10+13
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia. it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Optimal dilution of the Cytokeratin 10 + 13 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
Sterile Solution PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/15/2019