Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C188534-100 100 µg (1 mg/ml) $285 
LS-C188534-0.2 0.2 mg $405 
Antibody

Cytochrome B245 Heavy Chain Antibody (clone CS9) LS‑C188534

Cytochrome B245 Heavy Chain Antibody (clone CS9) LS‑C188534

Antibody:
Mouse Monoclonal to Human Cytochrome B245 Heavy Chain
Application:
IHC-Fr, WB, IP, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$285 
LS-C188534-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Cytochrome B245 Heavy Chain
Application:
IHC-Fr, WB, IP, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Cytochrome B245 Heavy Chain antibody LS-C188534 is an unconjugated mouse monoclonal antibody to human Cytochrome B245 Heavy Chain. Validated for ELISA, Flow, IHC, IP and WB.
Target
Human Cytochrome B245 Heavy Chain
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
CS9
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC - Frozen
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:10 - 1:100)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Cytochrome B245 solubilized in octyl-beta-gluycopyranoside.
Specificity
Recognizes the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterized by severe recurrent infections. The light chain (p22phox), along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. The core region of the CS9 epitope is amino acids 165 - 169 (KKPSE), a cytosolic epitope on p22phox not accessible on intact neutrophils. Clone CS9 also has an inhibitory effect on NADPH oxidase activation.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Reviews (0)

Featured Products

Cytochrome B245 Heavy Chain Antibody - Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human Cytochrome b-245 Heavy Chain . Permeabilised with Leucoperm
Species: Human
Applications: IHC - Frozen, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Thyrotropin / TSH ELISA Kit
Reactivity: Rat
Range: 185.2-15000 pg/ml
CD79A / CD79 Alpha Antibody - Anti-CD79A antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Species: Human, Mouse, Rat, Bovine, Guinea pig, Opossum, Rabbit, Chicken, Equine, Porcine
Applications: IHC, IHC - Paraffin, IHC - Frozen, Flow Cytometry
CD38 ELISA Kit
Reactivity: Human
Range: 0.94-60 ng/ml
HLA-E ELISA Kit
Reactivity: Human
Range: 6.25-400 pg/ml

Requested From: United States
Date Requested: 10/16/2019