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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C59281-100 100 µl (85 mg/ml) $425 
CYTIP Antibody - Anti-Cybr Antibody - Western Blot. Western blot of anti-Cybr antibody shows detection of recombinant Cybr using anti-HA tag antibody after immunoprecipitation using anti-Cybr. Lane 5 contains lysate from control HEK293 cells. Lane 6 shows IP/WB of HEK293 cells expressing Cybr-HA. Lane 7 and 8 are loaded similarly to lanes 5 and 6 but were immunoprecipitated with anti-HA. Lane 9 contains marker. Lane 10 contains purified CybrHA. Personal Communication, V. Coppola, NCI, Bethesda, MD.

Polyclonal CYTIP Antibody (C‑Terminus, WB) LS‑C59281

Polyclonal CYTIP Antibody (C‑Terminus, WB) LS‑C59281

Antibody:
Rabbit Polyclonal to Mouse CYTIP
Application:
WB, IP, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C59281-100
100 µl
85 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse CYTIP
Application:
WB, IP, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CYTIP antibody LS-C59281 is an unconjugated rabbit polyclonal antibody to CYTIP from human, mouse and rat. Validated for ELISA, IP and WB.
Target
Mouse CYTIP
Synonyms
CYTIP | B3-1 | CASP | Cbp HE | CYTHIP | Cytohesin binder and regulator | Cytohesin binding protein HE | Cytohesin-binding protein HE | CYBR | Cytohesin-interacting protein | HE | PSCDBP
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:10000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Immunogen
PSCDBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the C-terminal portion of mouse Cybr.
Epitope
C-Terminus
Specificity
A BLAST analysis was used to suggest cross-reactivity with Cybr from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Usage
This antibody has been tested for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kD in size corresponding to Cybr by western blotting after IP in the appropriate cell lysate or extract. To date, this antibody has not shown direct detection of endogenous or over-expressed Cybr protein, but is capable of immunoprecipitating the antigen from lysates. It is possible, but has not yet been confirmed, that this antibody recognizes a conformational epitope.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYTIP
O60759 NM_004288 NP_004279.3

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Requested From: United States
Date Requested: 2/24/2020